Fig. 3. (A) Co-localization of JAM-1 and actin (phalloidin staining) at the apical pole (arrows) in compact eight-cell embryos. (B) JAM-1 localization in representative eight-cell embryos at 0 hours, 3 hours, 6 hours and 9 hours after division in z-axis series (z) and single mid-plane (mp) sections. Bar, 20 µm. (C) Timing of cell-cell contact and apical polar staining of JAM-1 in eight-cell embryos at different times during the fourth cell cycle (n=30-40 embryos per time point; experiment repeated four times).