JCS -- Thomas et al. 117 (23): 5599 Figure IG5

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Fig. 5. (A) Localization of PKC isoforms ${zeta}$ , ${iota}$ / ${lambda}$ and ${delta}$ within representative compact eight-cell embryos (C8). Arrows indicate immunostaining at the apical membrane domain of polarized blastomere for PKC ${zeta}$ and ${delta}$ . (B) Immunolocalization of nectin-2 (arrows) within representative compact eight-cell embryo (C8), 16-cell morula (16-c) and blastocyst (Blast; left, mid-plane showing staining at trophectoderm junctions and at ICM cell contacts; right, z-axis series showing trophectoderm in tangential plane; Bl, blastocoel). n=10-20 embryos per stage or treatment in three replicates. Bar, 20 µm.