JCS -- Lin et al. 117 (23): 5609 Figure IG7

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 7. TR3 with a deletion of its DBD is able to induce apoptosis. (A) Schematic representation of TR3 and its mutants. (B) Cellular localization of GFP-TR3 ${Delta}$ DBD and GFP-TR3 in MGC80-3 cells. Cells were transfected with GFP-TR3 ${Delta}$ DBD or GFP-TR3 respectively and immunostained with anti-Hsp60 antibody followed by Cy3-conjugated secondary antibody to visualize mitochondria. GFP-TR3 ${Delta}$ DBD or GFP-TR3 and Hsp60 were visualized with a confocal microscope. (C) Effect of TR3 ${Delta}$ DBD on apoptosis induction. MGC80-3 cells were transfected with GFP-TR3 ${Delta}$ DBD or GFP-TR3, and then stained with DAPI to show nuclear morphology. Apoptotic cells were determined as described in Fig. 6B. GFP was used as a control.