Fig. 2. Role of fkh2⁺ in M/G1-specific transcription. (A) fkh2⁺, ace2⁺ and slp1⁺ are periodically expressed during M-phase. cdc25-22 (GG 308) cells were synchronised by transient temperature arrest and samples taken every 15 minutes upon release to the permissive temperature. RNA was analysed by northern blot using fkh2⁺, slp1⁺, ace2⁺, cdc15⁺, spo12⁺, fin1⁺ and adh1⁺ as probes, the latter as a loading control. Quantification of each transcript against adh1⁺ is shown. Septation indices were counted microscopically and are plotted to indicate the synchrony of the culture. (B) Loss of M/G1 periodic transcription in fkh2 cells. cdc25-22 (GG 308) and fkh2 cdc25-22 (JM 2285) cells were synchronised by transient temperature arrest and samples taken every 15 minutes upon release to the permissive temperature. RNA was analysed by northern blot using slp1⁺, ace2⁺, cdc15⁺, spo12⁺, fin1⁺ and adh1⁺ as probes, the latter as a loading control. Quantification of each transcript against adh1⁺ is shown. Septation indices were counted microscopically and are shown in black (fkh2 cdc25-22) or grey (cdc25-22).