Fig. 3. fkh2⁺ and sep1⁺ share an overlapping function. (A) sep1 rescues the lethal phenotype caused by overexpression of fkh2⁺. pREP3X:fkh2⁺ and pREP3X were transformed into wild-type and sep1 cells, and colonies streaked on EMM agar to induce overexpression of fkh2⁺. Micrographs of wild-type and sep1 cells overexpressing fkh2⁺, grown on EMM agar. Bar, 30 µm. (B) Loss of periodic transcription in sep1 cells. sep1 cdc25-22 (A 62) cells were synchronised by transient temperature arrest and samples taken every 15 minutes for northern blot analysis after release to the permissive temperature. The blot was probed consecutively with fkh2⁺, slp1⁺, ace2⁺, cdc15⁺, spo12⁺, fin1⁺ and adh1⁺ probes, the latter as a loading control. Quantification of each transcript against adh1⁺ is shown.