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Fig. 4. Transplanted eGFP-MSCs are circulating and/or trapped in the different tissues. Immnunohistochemistry revealed the presence of infused eGFP-MSCs in different tissues. Circulating or trapped (arrows to brown cells) MSCs were found in the spleen (A), gut (B) and lung (C). In the lung, trapped cells stained neither for {alpha}-sma [(red-pink) indicated by the right-hand arrow] (C) nor for the lectin Lycopersicon esculentum-Cy3 (D, overlay with eGFP). Trapped eGFP-MSCs have caused severe lung damage in some of the mice. Representative sections of an undamaged lung (E) and severely damaged lung (F) from two different mice that were injected with eGFP-MSCs (brown cells) are shown. Sections were counterstained with hematoxylin (A-C,E,F) or DAPI (D). Magnifications: x25 (E,F); x100 (C); x200 (D); x400 (A,B).