Fig. 5. Multiple-daughter formation in T. gondii. Phase and epifluorescence time-lapse images of a vacuole containing nine mitotic parasites (V1) and a second vacuole containing four interphase parasites (V2). Two parasites in the first vacuole are forming four daughters each (red and yellow ovals), leading eventually (40 minute time point) to a vacuole with 22 parasites.