Fig. 2. Poly(A)⁺ RNA in the nucleus and cytoplasm. Little poly(A)⁺ RNA is found at the nuclear periphery in a mouse fibroblast (NIH 3T3). Poly(A)⁺ RNA was detected by in situ hybridization using biotinylated poly(dT)₅₄ and streptavidin conjugated with Alexa594 (pseudo-coloured green); DNA was counterstained with DAPI (blue). Image kindly provided by Meg Byron, John McNeil and Jeanne Lawrence. Bar, 10 µm.