Fig. 3. The nuclei (but not cytoplasm) of permeabilized HeLa cells exclude a 40 kDa dextran conjugated with fluorescein isothiocyanate (FITC). Hela cells were permeabilized in saponin in a `physiological' buffer, washed in the same buffer, incubated (15 minutes, 27.5°C) in the precursors required for translation (as for Fig. 2 of Iborra et al., 2001), a 40 kDa dextran-FITC added, and cells imaged on a confocal microscope. FITC fluorescence is illustrated. Bar, 10 µm.