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Fig. 3. Transcytosis of flagellin does not correlate with bacterial movement. (A,B) T84 model epithelia were colonized apically with 5x108 wild-type S. typhimurium or isogenic fliD mutant. At indicated time, basolateral media was assayed for CFU (A) and western blots were probed for flagellin (B). (C) IL-8 secretion was measured after 5 hours.