Fig. 8. Anti-ß₁-integrin does not activate Bid, the release of cytochrome c and caspase-9 in c-FLIP_L overexpressing keratinocytes. c-FLIP_L HaCaT cells and mock infected cells were cultivated in the presence of anti-ß₁-integrin or irrelevant IgG antibody. Proteins were extracted from the full cell lysate for caspase-8, caspase-9 and Bid (A), whereas they were extracted from mitochondria and cytosol for cytochrome c (B). Western blot was performed with anti-procaspase-9, anti-procaspase-8, anti-Bid and anti-cytochrome c antibodies. ß-actin was used to normalize protein content.