Fig. 8. 3THDII is important for Myo3A tail localization to inner segment actin filament bundles. (A) The Myo3A tail fusion protein (GFP:Myo3AT, green) co-localized with inner segment actin filament bundles (stained red with phalloidin) as well as being localized to the rod inner segment cytosol (IS) and nuclei (N). (B) Elimination of 3THDII from the Myo3A tail fusion protein (GFP:Myo3AT3THDII) resulted in fluorescence being confined to cytosolic compartments of rods and not inner segment actin filament bundles. Instead, fluorescence is located in the rod connecting cilium (arrow), inner segment, and synapse but is excluded from the outer segment (OS) and nucleus (N, labeled in blue with DAPI). (C) Fluorescence from a GFP:3THDII fusion protein in inner segment actin filament bundles, inner segment cytosol and nuclei of rods. RPE, retinal pigmented epithelium. Scale bar: 10 µm.