Fig. 5. Construction of transgenic (Tg) mice expressing MOR23 in the testis under the control of the calmegin promoter. (A) The transgene used to generate the Tg mouse line. The arrows show the primers used for genome PCR analysis. (B) Genome PCR analysis with primer pairs I and III demonstrate the insertion of transgene. PCR products with primer pairs II and III were derived from endogenous MOR23. (C) In situ hybridization using DIG-labeled antisense MOR23 probe (MOR23 AS) revealed high-level expression of the MOR23 transgene in Tg mouse testis. Hybridization signals were detected without the TSA system. Calmegin antisense probe (Clgn AS) was used as a positive control. WT, wild-type mouse. Scale bar, 100 µm.