Fig. 2. Mutant BRG1 causes an increase in the size of the cell and the nucleus. (a) Image of a CSFE-labeled cell (left) and the corresponding DIC image (right). Bar, 10 µm. (b) Distribution of cell surface attachment area (in µm²) for B05-1 cells grown in the presence (n=65) or absence (n=61) of tetracycline for 3 days. Cells were at 20-25% confluence to permit examination of individual cells. (c) Distribution of cell volume in (in µm³) for B05-1 cells grown to 20-25% confluence in the presence (n=60) or absence (n=56) of tetracycline for 3 days. (d,e) B05-1 cells grown in the presence (d) or (e) absence of tetracycline for 3 days were Papanicolaou stained to examine the appearance and size of the nucleus. Bar, 10 µm.