Fig. 3. Cell attachment response to actin depolymerization and myosin II inhibition. Cell-substrate attachment is influenced by lat-A but not by blebbistatin. (A) Before treatment, myotubes are fused and firmly attached to the substrate. (B,C) Control cells and cells incubated in 50 µM blebbistatin for 20 minutes exhibit no visually observable change. (D) After a 20 minute incubation in 5 µM lat-A, however, cells bleb and self-peel off the substrate. The dotted lines indicate the location of the collagen-patterned strip in the IPN. Bars, 20 µm.