Fig. 2. Overexpression of GCC185 leads to the formation of GCC185 labelled tubular structures throughout the cytoplasm. COS cells were transfected with myc-GCC185, fixed with glutaraldehyde and processed for cryoelectron microscopy. Ultrathin cryosections were labelled with monoclonal antibodies to myc. Antibodies were detected with 10 nm protein A gold particles (c-myc 10 nm). Note the labelling on tubulo-vesicular structures (arrows) scattered throughout the cytoplasm that get close to the plasma membrane (B). G, Golgi; mc, mitochondria; pm, plasma membrane. Bars, 200 nm.