Fig. 4. GCC185 is recruited to structures distinct from the other three TGN golgins. (A) HeLa cells were transiently transfected as indicated, with either GFP-GCC185 or myc-GCC185, fixed, permeabilized and co-stained for endogenous golgin-97, p230 or GCC88. Golgin-97 was detected with a mouse monoclonal antibody and Alexa 568 goat anti-mouse IgG, p230 detected with human anti-p230 antibodies and Alexa 594 goat anti-human IgG and GCC88 detected with rabbit anti-GCC88 antibodies followed by Alexa 594 goat anti-rabbit IgG. (B) COS cells were transfected with myc-GCC185 and untagged GCC88, fixed, permeablized and stained with rabbit anti-GCC88 followed by Alexa 568 goat anti-rabbit IgG, and anti-myc monoclonal antibody followed by FITC goat anti-mouse Ig. Shown are images of transfected cells expressing high levels of both myc-GCC185 and GCC88. Bar, 10 µm.