Fig. 5. The TGN resident membrane protein, sialyltransferase, segregates into GCC185-labelled structures. HeLa cells, stably expressing SialylT fused to the VSV-G epitope, were transiently transfected with (A) GFP-GCC185 or (B) GFP-GCC88, fixed, permeabilized and stained for VSV-G epitope tagged SialylT with mouse monoclonal antibodies and Alexa 568 anti-mouse IgG. Superimposed images (merge) are shown. Arrrows in A indicate structures in the periphery of the cell, labelled with both GFP-GCC185 and SialylT. Bar, 10 µm.