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Fig. 8. Invariant tyrosine of the GRIP domain is not required for Golgi targeting of GCC185GRIP. (A) DNA sequence profile of GFP-GCC185GRIP with the TAC coding for the invariant tyrosine highlighted, and an image of COS cells transfected with GFP-GCC185GRIP. (B) DNA sequence profile of GFP-GCC185GRIP (Y4A) showing the GCA substitution (boxed), with an image of COS cells transfected with GFP-GCC185GRIP (Y4A). Bars, 20 µm.