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Fig. 2. Identification of motifs in the G3 domain interacting with PSGL-1. (A) Constructs containing different combinations of versican G3 domain motifs used for yeast two-hybrid assays. EGF, epidermal growth factor domain; CRD, carbohydrate recognition domain; CBP, complement binding protein domain. (B) Filter membrane binding assay of recombinant G3 constructs with PSGL-1. EGF-containing constructs interacted with PSGL-1.