Fig. 5. The effects of mini-versican and the G3 motifs on cell aggregation. (A) Mammalian expression constructs of mini-versican, G3, CRDCBP and CBP were generated as shown. (B) Expression of these constructs in U87 cells was confirmed by western blotting with 4B6 using culture medium from transfected cells. (C) Culture medium containing mini-versican or G3-induced aggregation of cells transfected with PSGL-1 or PSGL(279-402) construct (n=3; ^**P<0.01). (D) The A2058 cells stably transfected with PSGL-1 were incubated with culture medium collected from U87 cells transfected with the G3 construct at different dilutions for different time periods. The aggregation effects were reduced as G3 was diluted.