Fig. 4. Relative positions of human chromosome-12 and chromosome-16 territories (CT12 and CT16, respectively). (A) The definition of a representative peak point for a CT. Green dots indicate the fluorescence peak center (FPC). The location of the CT is represented by the position of its FPC. (B) Schema of the minimum FPC distance. We measured the pixel-to-pixel distance between the FPCs of CT12 and CT16, and then recorded the shortest of the four distances obtained from the four possible pairings as the minimum FPC distance. (C) Quantitative evaluation of the minimum FPC distance between CT12 and CT16. The distances are normalized using the radius of the standardized 2D nucleus. The significance of the difference between minimum FPC distances in preadipocytes and adipocytes was evaluated using the Welch's t test (P=0.017).