Fig. 1. Type IV collagen does not localize in the basement membrane (BM) regions of Hsp47^–/– mutant mouse embryos at 9.5 days post coitus (dpc). (A-F) Wild-type (WT) and Hsp47^–/– embryos were fixed with paraformaldehyde. Neighboring cryosections of the embryos were then analysed by staining with haematoxylin and eosin (A,B) and by immunostaining with anti-Hsp47 antibody and FITC-conjugated second antibody (C,D) or anti-type IV collagen antibody and rhodamine-conjugated second antibody (E,F). Arrows indicate BM regions. ME, mesenchyme; EP, epithelium. Bar, 50 µm.