Fig. 10. CHOP is upregulated in Hsp47^–/– mutant mouse embryos at 10.5-11.0 dpc. The levels of CHOP (A), Hsp47 (B) and ß-actin (C) mRNAs in wild-type and Hsp47^–/– embryos at 9.5, 10.5 and 11.0 dpc were analysed by RT-PCR using specific primers. CHOP mRNA levels were elevated in Hsp47^–/– embryos at 10.5 and 11.0 dpc, but not at 9.5 dpc.