Fig. 3. Type IV collagen does not localize in the BM regions of Hsp47^–/– mutant mouse embryos at 10.5 dpc. (A-E) Neighboring sections of wild-type and Hsp47^–/– embryos that included the neural tube region were stained with haematoxylin and eosin (A,B) or with antibodies against Hsp47 (C,D) or type IV collagen (E,F). Arrows indicate BM regions. Bar, 50 µm.