Fig. 4. Staining with anti-laminin and anti-nidogen antibodies reveals that the BM of Hsp47^–/– embryos at 10.5 dpc is fractured. (A-E) Neural tube regions of wild-type and Hsp47^–/– embryos were analysed for the immunolocalization of laminin (A,B), nidogen-1 (C,D) and fibronectin (E,F). Arrows indicate BM regions. Bar, 50 µm.