Fig. 2. The localization of H3V shifts dramatically over the cell cycle. A procyclic-form cell line (PFJEL12) expressing TY1-H3V (green) from its endogenous locus was subjected to indirect immunofluorescence, stained with DAPI to detect DNA (blue) and examined by deconvolution microscopy. (A) In interphase cells, TY1-H3V is often present as discrete foci at the nuclear periphery. (B) As the cell enters mitosis, TY1-H3V moves to the center of the nucleus. (C) As mitosis proceeds, TY1-H3V heads towards the poles. (D) At the end of mitosis, TY1-H3V remains at the poles before dispersing along the nuclear periphery. The region within the nucleus that is not stained by DAPI corresponds to the nucleolus as verified previously (Das et al., 1998). n, nucleus; k, kinetoplast. Bar, 2 µm.