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Fig. 4. H3V associates with mini-chromosomes. (A-D) Indirect-immunofluorescence of TY1-H3V (green) was combined with detection of mini-chromosomes by FISH using a probe specific for the 177-bp repeats (MC177, red). DNA (blue) was detected with DAPI. All cells are procyclics (PFJEL12) at (A) interphase, (B,C) mitosis and (D) late mitosis. Arrowheads indicate trailing TY1-H3V foci that do not overlap with 177-bp repeats. Bar, 2 µm.