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Fig. 3. Quantification of cortical granule exocytosis (CGE) at fertilization: input of G-proteins. (A) Increase in FM1-43 fluorescence indicates CGE. (B) G{alpha}i inhibitor (competitor peptide; 50 µM final concentration in egg) changes exocytosis dynamics. (C) G{alpha}s inhibitor (inhibitory antibody; 100 µg/ml final concentration in egg) decreases exocytosis. (D) G{alpha}q inhibitor (competitor peptide; 50 µM final concentration in egg) decreases exocytosis. Each data point represents an average of 3-6 recordings (error bars, 1±s.d.).