Fig. 4. Inhibitors of Gs- and Gq-mediated signaling interfere with normal Ca²⁺ release at fertilization. (A) Ratiometric quantification of normal Ca²⁺ release at fertilization. (B) Anti-Gs (100 µg/ml final concentration in egg) and Ca²⁺ release at fertilization. (C) Gq peptide (50 µM final concentration in egg) and Ca²⁺ release at fertilization. Each data point represents an average of 3-8 recordings (error bars, 1±s.d.). (D) Ca²⁺ wave in a control egg (top left; pseudocolored); compare with the abnormal pattern in a Gq peptide-injected egg (bottom right, observed in 42.5% of eggs). The images were independently adjusted to better reveal the features (wave versus spikes); colors reflect normalized fluorescence ratios of individual pixels (see side bars).