JCS -- Luciani et al. 117 (25): 6019 Figure IG1

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Fig. 1. Replication kinetics in Xenopus egg extract. (A) Sperm nuclei were incubated at 12 ng DNA µl^-1 in interphase Xenopus egg extract supplemented with [ ${alpha}$ -³²P]dATP. At the indicated times, total DNA synthesis was measured. (B) Sperm nuclei were incubated in extract at 12 ng DNA µl^-1. At the indicated times, samples were pulse labelled with [ ${alpha}$ -³²P]dATP for 2 minutes. DNA was separated on an alkaline agarose gel and autoradiographed. The migration of end-labelled ${lambda}$ -phage HindIII-digested DNA is also shown.