Fig. 1. Actin depolymerization does not affect the Can1p distribution in the plasma membrane. Cells expressing CAN1GFP (strain KM30; A-E) were treated with latrunculin A, an inhibitor of actin polymerization (F-J). Differential interference contrast (DIC) images (A,F), fluorescence signals from the corresponding transversal sections (B,G) and surface confocal sections (C,H) are presented. The treatment efficiency was tested by TRITC-phalloidin staining of actin filaments in fixed cells (fluorescence in D,I; DIC in E,J). Notice the absence of the fluorescence signal from I. The identical scanning parameters were set during the acquisition of D and I. Bars, 10 µm (A); 5 µm (E,J).