Fig. 3. Microtubules are not required to maintain the Can1p distribution. Cells expressing CAN1GFP (strain KM30; A-E) were treated with nocodazole in order to disassemble the microtubules (F-J). Differential interference contrast (DIC) images (A,F), fluorescence signals from the corresponding transversal confocal sections (B,G) and surface confocal sections (C,H) are presented. The treatment efficiency was tested by indirect immunofluorescence detection of tubulin in fixed cells (fluorescence in D,I; DIC in E,J). Notice the absence of the fluorescence signal from I. The identical scanning parameters were set during the acquisition of D and I. Bar, 10 µm.