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Fig. 4. Can1p plasma-membrane distribution is not related to the cell wall. Cells expressing CAN1GFP (strain KM30) were protoplasted with zymolyase. Differential interference contrast images (A,B,G,I), fluorescence signals from the corresponding transversal confocal sections (C,D), and surface confocal sections (E,F,H) before (A,C,E) and after (B,D,F-I) the treatment are presented. Bar, 10 µm.