Fig. 5. Pma1p and Sur7p plasma-membrane distributions in various protoplasts. Cells expressing Pma1GFP (top left) or Sur7p (bottom left) were protoplasted by a consecutive treatment with zymolyase (middle) and glucuronidase (right). Differential interference contrast images (AC,J-L), fluorescence signals from the corresponding transversal confocal sections (D-F,M-O) and surface confocal sections (G-I,P-R) are presented. Arrowheads in C,I point to a burst cell. Bars, 10 µm.