Fig. 6. Dynamics of Pma1GFP in the plasma membrane. A round spot of 0.5 µm diameter was bleached within a surface optical section of a living cell (strain KM12) and the fluorescence recovery was measured. Fluorescence intensities were corrected for the photobleaching caused by the repeated scans of the sample and normalized to the intensity before the bleaching (level 1 in the graph). Mean values of the relative fluorescence intensities in nine experiments (dots) and the fitted curve are presented.