Fig. 1. Generation and characterization Scrib domain constructs. (A) Schematic representation of the seven different Scrib:myc proteins used in this study. LRR repeats (green ovals), LAPSDa (red oval), LAPSDb (red square), PDZ domains (blue boxes) and C-terminal region (red line) are indicated. FL is the full-length Scrib; CT lacks the C-terminal region; LRR contains only the N-terminal LRR region; PDZ lacks the central PDZ region; PDZ contains only the central PDZ region; LRR lacks the N-terminal LRR region; CT contains only the C-terminal region. The numbers above each line represent the amino acids present at the beginning or end of each domain. All constructs carry six myc tags (white circle). (B) Western blot showing the size and stability of Scrib:myc proteins in vivo. Embryos expressing each Scrib:myc proteins (progeny of Sca-Gal4 x UAS-scrib:myc crosses) were homogenized, run in the indicated lanes and the western blot was probed with a Myc antibody.