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Fig. 3. Scrib domain regulation of Mira basal localization in stage 15 neuroblasts. Top row, Mira localization in metaphase neuroblasts; middle row, the same neuroblasts showing both Mira (green) and the mitotic DNA marker phosphohistone H3 (red); lower row, quantification of the Mira localization phenotype: normal basal crescent, black bar; uniform cortical localization, crosshatched bar; uniform cortical plus cytoplasmic and spindle-associated, blue bar; apical up for all images. Scrib domain abbreviations are given in Fig. 1. (A) Wild-type neuroblast showing normal Mira basal crescent. (B) scrib1/scrib1 neuroblast showing cytoplasmic and spindle-associated Mira. (C-I) scrib1/scrib1 embryos expressing the indicated Scrib:myc transgene (progeny from UAS-scrib:myc x worniu-gal4). (C,D) FL and {Delta}CT proteins provide full rescue of basal Mira localization. (E,F) LRR and {Delta}PDZ result in predominantly uniform cortical Mira localization or cortical with weak basal enrichment. (G-I) PDZ, {Delta}LRR and CT proteins give no rescue of Mira cortical or basal localization.