Fig. 5. Scrib domain regulation of mitotic spindle and cell size asymmetry in neuroblasts. Stage 15 telophase neuroblasts (identified as Pins⁺ and Mira⁺) were scored for cortical markers, spindle morphology, and cell size. Schematic of the phenotypic categories is shown at the top: apical cortex, blue (Pins); basal cortex, red (Mira); mitotic spindle and centrosomes, green (-tubulin). Cell size ratios are apical/basal and are defined as: normal, >1.25; symmetrical, 0.8-1.25; inverted, <0.8. Wild-type neuroblasts divide asymmetrically. scrib¹/scrib¹ neuroblasts show only 65% asymmetric divisions. scrib¹/scrib¹ neuroblasts expressing FL or CT proteins rescue normal asymmetric divisions. scrib¹/scrib¹ neuroblasts expressing LRR, PDZ, PDZ, LRR, or CT proteins fail to rescue asymmetric cell division.