Fig. 3. Electron microscopy of lamin-induced intranuclear membrane structures. (A-E) EM sections of isolated oocyte nuclei are shown. Oocytes were injected with RNA encoding lamin B2 (A-D) or lamin B2-SaaX (E). Nuclei were isolated and processed for electron microscopy. Sections were stained with uranyl acetate and lead citrate (A-C,E) or processed for pre-embedding immunoelectron microscopy (D). Lamin B2 was detected with mAb L7-8C6 and nanogold-coated secondary antibody. Arrows in A indicate membrane arrays attached to the nuclear envelope. Arrows in D indicate gold particles decorating the outer membrane cisternae of a lamin B2-induced membrane array. C, cytoplasm; NC, nucleoplasm; NE, nuclear envelope; NPC, nuclear pore complex. Bar, 5 µm (A); 1 µm (B,E); and 0.5 µm (C,D).