Fig. 1. Schematic of GFP-lamin fusion proteins. These are wild-type human lamin A, Xenopus lamin B2, Drosophila lamins Dm0 and C, mutants derived from these lamins and an N-terminal deletion mutant of zebrafish lamin B2 (zB2-N3). The position of the GFP, the helical region (1A, 1A, 2), the nuclear localization signal (NLS) and the CxxM-motif are indicated. Three N-terminal deletion mutants (B2-N1, B2-N2, B2-N3) and a point mutation (B2-SxxM) where the cysteine in the CxxM-motif had been replaced by a serine have been generated from the Xenopus lamin B2. C-CxxM is a Drosophila lamin C mutant containing the CxxM motif of lamin Dm0 and C-CxxM-N1, a Drosophila lamin C mutant with the CxxM motif lacking the N-terminal 228 amino acids. The numbers of amino acids (AA) of each lamin contained in the GFP fusion protein are listed.