Fig. 1. LAP2 dynamics during nuclear assembly. (A-D) HeLa cells stably expressing YFP-LAP2 were transiently transfected with constructs encoding CFP-histone 2B and analyzed by live cell imaging. Confocal images of cells in interphase and metaphase (A) or at various stages during anaphase to G1 phase progression (B) or anaphase (C) are shown. Arrowheads indicate LAP2 at the tips of chromatin extensions; arrows indicate outer core regions. See also Movie 1 in supplementary material. (D) Isosurface reconstruction of z-stacks of images. Non-chromosome-associated clusters were excluded from the isosurface reconstruction. Numbers in images indicate elapsed time from anaphase onset (B) or from first image (D). Bars, 10 µm.