Fig. 6. LAP2 interacts with BAF. (A) In vitro translated BAF (input) was mixed with buffer (-), recombinant full length LAP2 (1-693) or LAP2 C-terminal fragment (410-693) and complexes were precipitated with immobilized monoclonal antibodies to LAP2. Panels show immunoblots using antibodies to BAF (upper) and to LAP2 (lower). (B) LAP2 was immunoprecipitated from metaphase cell lysates. Controls were performed in the absence of antibodies. Supernatant (S) and pellet (P) fractions were analyzed by immunoblotting using antiserum to BAF and to LAP2. IgG, immunoglobulin heavy chain.