Fig. 10. Taxol displaces tau from microtubules in vitro. CHO cells transfected with PcDNA3 (lanes 1 and 1'), wild-type tau (lanes 2 and 2') or GFP-tau (lanes 3 and 3') were homogenized and the supernatants were subjected to conditions allowing the polymerization of tubulin in vitro in the absence (lanes 1,2,3) or presence (lanes 1',2',3') of taxol. Microtubule pellets were examined by SDS-PAGE as described in Materials and Methods. Tau protein and GFP-tau were detected with a polyclonal antibody against the C-terminus of tau (A). The same blots were developed with antibody against -tubulin (B).