Fig. 7. Brief incubation with taxol does not significantly affect the MT array. Fluorescence micrographs of the same fibroblast-like cell expressing GFP-tau and loaded with Cy3-tubulin before (upper panel) and 3 minutes after (lower panel) the onset of incubation with 1 µM taxol. Exposure to taxol induced rapid retraction of the leading edge and significant change in the overall shape of the cell. Single MTs before exposure to taxol can be seen both in the GFP channel (A) and in the Cy3 channel (B). After incubation with taxol (D), Cy3-labeled MTs are largely intact (arrows). No GFP-tau-labeled MTs can be detected after taxol incubation (C).