Fig. 4. Suppression of the echinocandin-hypersensensitive growth phenotype of the ehs2-1 mutant by overexpression of rho1⁺. MS38 (rgf3⁺) was transformed with pREP3X (empty vector) and GI 1 (ehs2-1/rgf3) was transformed with pREP3X-rho1 (rho1⁺), pREP3X-rho2 (rho2⁺), pREP3X-rho3 (rho3⁺), pREP3X-rho4 (rho4⁺), pREP3X-rho5 (rho5⁺), pREP3X-cdc42 (cdc42⁺) and pREP3X (empty vector). Transformants were streaked onto MM, MM plus thiamine, MM plus 1.5 µg ml^-1 echinocandin and MM plus thiamine and 1.5 µg ml^-1 echinocandin plates, and incubated at 32°C for 4 days.