Fig. 7. Rgf3p localizes to the septum. (A) Wild-type cells were transformed with an integrative plasmid expressing EGFP-rgf3⁺ under its own promoter (strain VT128). Cells were grown at 28°C. (Top) EGFP-Rgf3p localization in a population of living cells at different stages of the cell cycle is shown in the centre. The corresponding differential interference contrast images are shown on the left and Calcofluor White (Cfw) staining is shown on the right. (Bottom) A square with cells that had not yet developed a visible septum is enlarged. These cells already showed EGFP-Rgf3p dots of fluorescence. (B) The rgf3 mRNA levels were followed by northern-blot analyses in a cdc25-22 block-release experiment. RNA samples were collected every 20 minutes along two consecutive cell cycles after release to 25°C. The blot was probed for rgf3 and for act1, the latter as an mRNA loading control.