Fig. 2. Effect of exogenous 4.1R expression on the distribution of the microtubule nucleating proteins -tubulin and pericentrin. Cells were triple labeled to detect exogenous 4.1R⁶⁰16,18, tubulin and -tubulin (top) or exogenous 4.1R⁶⁰16,18, tubulin and pericentrin (bottom). Arrows and asterisks indicate transfected and untransfected cells, respectively. Images are projections of x-y optical section stacks acquired by confocal microscopy. Bar, 20 µm.