Fig. 3. GFP-PTEN remains evenly distributed during chemotaxis. (A) HL60 cells co-expressing GFP-PTEN and RFP were analyzed in chemotaxis assays towards fMLP. Dark-phase, green fluorescence, and red and green channel merge are shown; see supplementary material, Movie 3. The asterisk indicates the pipette tip. The cell is representative of 14 out of 15 cells analysed. Bar, 10 µm. (B) Red and green fluorescence scanning of cells in panel A.