Fig. 5. PTEN chimeras are found mostly in the cytosol of Jurkat cells. Unstimulated or CXCL12 (100 nM)-stimulated mock-, PTEN-GFP- and PTEN-C/S-GFP-expressing Jurkat cells were fractionated in density gradients to isolate membrane (M) and cytosolic (C) fractions, and western blots were performed with anti-PTEN, -p85 PI3K, -transferrin receptor (TfR) and -tubulin antibodies (n=3). Relative accumulation of p85 and PTEN-GFP in the membrane and cytosolic fractions during a CXCL12 stimulation time course was calculated by densitometry.